目的 建立甘肃省肉苁蓉、沙苁蓉、盐生肉苁蓉、兰州肉苁蓉的超高效液相色谱(UPLC)指纹图谱及4种指标性成分含量测定,并进行肉苁蓉药材浸出物含量测定,为肉苁蓉药材的质量评价提供理论依据。方法 按照2020年版《中国药典》一部中的醇溶性浸出物下的冷浸法测定浸出物含量;采用色谱柱 ACQUITY UPLC® BEH shield RP 18(2.1 mm×100 mm,1.7 μm),流动相乙腈-0.1%甲酸水溶液梯度洗脱,体积流量0.1 mL·min-1,检测波长330 nm,柱温 35 ℃。结果 醇溶性浸出物含量为36%~68%;松果菊苷、肉苁蓉苷A、毛蕊花糖苷、2'-乙酰基毛蕊花糖苷在2 μg进样量与峰面积积分值线性关系良好(r≥0.997 2),RSD≤3.82%。结论 该方法操作简便、精密度高、结果可靠,可用于肉苁蓉、沙苁蓉、盐生肉苁蓉、兰州肉苁蓉的质量评价。
Abstract
OBJECTIVE To establish UPLC method for the determination of echinacoside, cistanchis glycosides A, verbascoside and 2'-acetyl verbascoside in Herbra Cistanches. METHODS The content of the extract was determined by cold immersion method under the alcohol-soluble extract in the first edition of Chinese Pharmacopoeia 2020. The chromatographic column ACQUITY UPLC BEH SHIELD RP 18 (2.1 mm×100 mm, 1.7 μm) was used. Mobile phase acetonitrile -0.1% formic acid aqueous solution gradient elution; volume flow rate of 0.1 mL·min-1. The detection wavelength is 330 nm. The column temperature is maitained at 35 ℃. RESULTS The content of alcohol soluble extract was 36%~68%. Echinacea glycosides, cistanchis glycosides A, 2'-acetyl verbascoside have a good linear relationship (r≥0.999 6) with RSD≤3.69%. CONCLUSION The method is simple to operate, high precision and reliable. UPLC can be used for simultaneous determination of four index components in Herbra Cistanches.
关键词
肉苁蓉 /
沙苁蓉 /
超高效液相色谱法 /
肉苁蓉苷A /
松果菊苷 /
毛蕊花糖苷
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Key words
C. deserticola /
C. sinensis /
UPLC /
Cistanche glycosides A /
Echinacoside /
Verbascoside
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中图分类号：
R284
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参考文献
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脚注
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基金
甘肃省教育厅高等学校创新能力提升项目资助(2020A-079);2018年全国中药资源普查工作项目资助
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